Difference between revisions of "Part:BBa K389413:Design"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K389413 short</partinfo> | <partinfo>BBa_K389413 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | sensitivity tuner to enhance the luciferase signal | + | a modified sensitivity tuner <partinfo>I746390</partinfo> to enhance the luciferase signal which interacts as a reporter for a ''vir'' promoter |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | * luciferase from pGL4.10luc2 (Promega) | + | * luciferase from pGL4.10luc2 (Promega) (<partinfo>K389004</partinfo>) |
| − | * | + | * terminator, RBS and constitutive promoter from the parts.igem (<partinfo>B0017</partinfo>, <partinfo>B0034</partinfo>, <partinfo>J23110</partinfo>) |
| − | * | + | * sensitivity tuner part consists inter alia of |
| − | * ''vir'' promoter from Agrobacterium tumefaciens C58, TI-plasmid | + | ** delta activator from phage phiR73 (<partinfo>I746352</partinfo>) |
| + | ** PF promoter from phage P2 (<partinfo>I746360</partinfo>) | ||
| + | * ''vir'' promoter from Agrobacterium tumefaciens C58, TI-plasmid (<partinfo>K389003</partinfo>) | ||
===References=== | ===References=== | ||
| + | [http://parts.mit.edu/igem07/index.php/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project] | ||
Revision as of 11:23, 17 October 2010
vir promoter + sensitivity tuner + luciferase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2455
Illegal NgoMIV site found at 3799
Illegal NgoMIV site found at 3820
Illegal AgeI site found at 1978
Illegal AgeI site found at 2090
Illegal AgeI site found at 2292
Illegal AgeI site found at 3523 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 3705
Design Notes
a modified sensitivity tuner BBa_I746390 to enhance the luciferase signal which interacts as a reporter for a vir promoter
Source
- luciferase from pGL4.10luc2 (Promega) (BBa_K389004)
- terminator, RBS and constitutive promoter from the parts.igem (BBa_B0017, BBa_B0034, BBa_J23110)
- sensitivity tuner part consists inter alia of
- delta activator from phage phiR73 (BBa_I746352)
- PF promoter from phage P2 (BBa_I746360)
- vir promoter from Agrobacterium tumefaciens C58, TI-plasmid (BBa_K389003)
References
[http://parts.mit.edu/igem07/index.php/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project]