Difference between revisions of "Part:BBa K389412:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K389412 short</partinfo> | <partinfo>BBa_K389412 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | sensitivity tuner to enhance the luciferase signal | + | a modified sensitivity tuner <partinfo>I746380</partinfo> to enhance the luciferase signal which interacts as a reporter for a ''vir'' promoter |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | * luciferase from pGL4.10luc2 (Promega) | + | * luciferase from pGL4.10luc2 (Promega) (<partinfo>K389004</partinfo>) |
| − | * | + | * terminator, RBS and constitutive promoter from the parts.igem (<partinfo>B0017</partinfo>, <partinfo>B0034</partinfo>, <partinfo>J23110</partinfo>) |
| − | * ''virG'' was synthesized by Mr. Gene | + | * sensitivity tuner part consists inter alia of |
| − | * ''vir'' promoter from | + | ** pag activator from phage PSP3 (<partinfo>I746351</partinfo>) |
| + | ** PF promoter from phage P2 (<partinfo>I746360</partinfo>) | ||
| + | * ''virG'' was synthesized by Mr. Gene (<partinfo>K389002</partinfo>) | ||
| + | * ''vir'' promoter from Agrobacterium tumefaciens C58, TI-plasmid (<partinfo>K389003</partinfo>) | ||
===References=== | ===References=== | ||
| + | [http://parts.mit.edu/igem07/index.php/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project] | ||
Revision as of 11:19, 17 October 2010
vir promoter + sensitivity tuner + luciferase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2428
Illegal NgoMIV site found at 3772
Illegal NgoMIV site found at 3793
Illegal AgeI site found at 1951
Illegal AgeI site found at 2063
Illegal AgeI site found at 2265
Illegal AgeI site found at 3496 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 3678
Design Notes
a modified sensitivity tuner BBa_I746380 to enhance the luciferase signal which interacts as a reporter for a vir promoter
Source
- luciferase from pGL4.10luc2 (Promega) (BBa_K389004)
- terminator, RBS and constitutive promoter from the parts.igem (BBa_B0017, BBa_B0034, BBa_J23110)
- sensitivity tuner part consists inter alia of
- pag activator from phage PSP3 (BBa_I746351)
- PF promoter from phage P2 (BBa_I746360)
- virG was synthesized by Mr. Gene (BBa_K389002)
- vir promoter from Agrobacterium tumefaciens C58, TI-plasmid (BBa_K389003)
References
[http://parts.mit.edu/igem07/index.php/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project]