Difference between revisions of "Part:BBa K329003"

Revision as of 16:30, 12 October 2010

Strong RBS (B0034) - Tetracycline resistance protein TetA(C) (J31007)

Tetracycline resistance protein (TetA(C) inner-membrane-associated protein BBa_J31007) with a Strong RBS upstream (BBa_B0034). However, overexpression of TetA(C) is detrimental to cell growth. We have designed several translational units for TetA(C) (see BBa_K329000, BBa_K329004).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 162
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 308
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 334
Illegal NgoMIV site found at 702
Illegal NgoMIV site found at 862
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K329003 short</partinfo>
-Tetracycline resistance protein (TetA(C) inner-membrane-associated protein <partinfo>BBa_J31007</partinfo>) with a Strong RBS upstream (<partinfo>BBa_B0034</partinfo>). However, overexpression of TetA(C) is detrimental to cell growth. We have designed several translational units for TetA(C) (see <partinfo>BBa_K329000</partinfo>)
+Tetracycline resistance protein (TetA(C) inner-membrane-associated protein <partinfo>BBa_J31007</partinfo>) with a Strong RBS upstream (<partinfo>BBa_B0034</partinfo>). However, overexpression of TetA(C) is detrimental to cell growth. We have designed several translational units for TetA(C) (see <partinfo>BBa_K329000</partinfo>, <partinfo>BBa_K329004</partinfo>).