Difference between revisions of "Part:BBa K389016"

Revision as of 19:00, 4 October 2010

VirA/G reporter device mRFP

This BioBrick contains a complete VirA/G receptor system with an mRFP (BBa_E1010) under the control of a vir promoter as reporter gene.

Usage and Biology

This BioBrick is for testing, characterizing and measuring the VirA/G signaling system. This system contains an unmutated virA, so it is possible to measure how the natural VirA/G system works and reacts. It is also possible to determine the basal transcription of the vir promoter and its activity after inducing the system with acetosyringone.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 647
Illegal NheI site found at 2581
Illegal NheI site found at 2604
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1632
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 4260
Illegal AgeI site found at 4372
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1768

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K389016 short</partinfo>
-This BioBrick is for testing the virA/G signaling system. The read-out is the mRFP <partinfo>E1010</partinfo>. This system contains an unmutated ''virA'', so it is possible to measure, how the natural virA/G system works and reacts.
+This BioBrick contains a complete VirA/G receptor system with an mRFP (<partinfo>E1010</partinfo>) under the control of a ''vir'' promoter as reporter gene.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This BioBrick is for testing, characterizing and measuring the VirA/G signaling system. This system contains an unmutated ''virA'', so it is possible to measure how the natural VirA/G system works and reacts. It is also possible to determine the basal transcription of the ''vir'' promoter and its activity after inducing the system with acetosyringone.
 <!-- -->