Difference between revisions of "Part:BBa I50041"

Latest revision as of 22:13, 27 September 2010

pSC101 origin of replication, reverse

pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids. This part is the reverse copy of BBa_I50042.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_I50041 short</partinfo>
-pSC101 origin of replication which is supposed to have low copy number.  This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site.  It is also the origin of replication in pSB4* series plasmids.  This part is the reverse copy of BBa_I50040.
+pSC101 origin of replication which is supposed to have low copy number.  This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site.  It is also the origin of replication in pSB4* series plasmids.  This part is the reverse copy of BBa_I50042.
 <!-- Add more about the biology of this part here