Difference between revisions of "Part:BBa J70628"

Revision as of 20:42, 11 August 2010

RFC12 Sce VMA Intein Internal Domain This is a RFC12 compatible Intein self-splicing protein. It is capable of self cleavage induced by overnight incubation at 4°C with 50 mM DDT.

Usage and Biology

Intein domains are commonly fused with CBD (BBa_J70627) and then used to cleave off the CBD tag after purification, yielding very high levels of the target protein. Note that the target protein's N terminal or C terminal residue can affect cleavage.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 486
Illegal BamHI site found at 1339
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1195
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_J70628 short</partinfo>
+This is a RFC12 compatible Intein self-splicing protein.  It is capable of self cleavage induced by overnight incubation at 4°C with 50 mM DDT.
-TODO
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Intein domains are commonly fused with CBD (<partinfo>J70627</partinfo>) and then used to cleave off the CBD tag after purification, yielding very high levels of the target protein. Note that the target protein's N terminal or C terminal residue can affect cleavage.
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