Difference between revisions of "Part:BBa I50052:Design"

 
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<partinfo>BBa_I50052 short</partinfo>
 
<partinfo>BBa_I50052 short</partinfo>
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This part is derived from BBa_I50042.  It contains a point mutation that changes Alanine to Valine in RepA protein.
 
This part is derived from BBa_I50042.  It contains a point mutation that changes Alanine to Valine in RepA protein.
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Generated using the following mutagenesis primers and the Stratagene QuickChange protocol:
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Mutation-forward: <code>5' cgttcacttataaccaatacgttcagatgatgaacatcagtag 3' </code>
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Mutation-reverse: <code>5' ctactgatgttcatcatctgaacgtattggttataagtgaacg 3' </code>
  
 
===References===
 
===References===

Latest revision as of 18:23, 21 June 2010

pSC101 temperature sensitive origin of replication


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

See design notes for Part:BBa_I50042.


Source

This part is derived from BBa_I50042. It contains a point mutation that changes Alanine to Valine in RepA protein.

Generated using the following mutagenesis primers and the Stratagene QuickChange protocol:

Mutation-forward: 5' cgttcacttataaccaatacgttcagatgatgaacatcagtag 3'

Mutation-reverse: 5' ctactgatgttcatcatctgaacgtattggttataagtgaacg 3'

References