Difference between revisions of "Part:BBa I52001:Design"

Revision as of 15:46, 10 March 2010

ccdB and nonfunctional pUC19 derived high copy origin

Design Notes

The BioBrick standard vectors are designed to be easy to use for their most common purpose: assembly of BioBrick standard biological parts. To meet this requirement, we included BBa_P1016 within the BioBrick cloning site. BBa_P1016 encodes the positive selection marker ccdB. Positive selection markers prevent one of the most common problems during assembly of BioBrick parts: contamination of the ligation reaction with uncut plasmid DNASambrook-2001. Any cells transformed with the uncut plasmid DNA produce the lethal protein ccdB and dieBernard-Gene-1994 Bernard-Gene-1995 Bernard-Biotechniques-1996. Note, however, that the drawback of this solution is that inclusion of this part requires that users propagate both the base vector and any derived vectors in E. coli strains tolerant of ccdB expression such as DB3.1Bernard-J-Mol-Biol-1992 Miki-J-Mol-Biol-1992.

Source

BBa_P1016 (ccdB positive selection marker) and BBa_I50020 (nonfunctional high copy origin derived from pUC19).

References

<biblio>

1. Bernard-J-Mol-Biol-1992 pmid=1324324
2. Miki-J-Mol-Biol-1992 pmid=1316444
3. Bernard-Gene-1994 pmid=7926841
4. Bernard-Gene-1995 pmid=7557407
5. Bernard-Biotechniques-1996 pmid=8862819

</biblio>

• US Patent Number 5,910,438 "Cloning and/or sequencing vector" Bernard P, Gabant P, University Libre de Bruxelles, 1999. [http://www.google.com/patents?vid=USPAT5910438 Google Patents]
• US Patent Number 6,180,407 B1 "Cloning and/or sequencing vector" Bernard P, Gabant P, Universit Libre de Bruxelles, 2001. [http://www.google.com/patents?vid=USPAT6180407 Google Patents]
• US Patent Number 7,176,029 B2 "Cloning and/or sequencing vector" Bernard P, Gabant P, Universit Libre de Bruxelles, 2007. [http://www.google.com/patents?vid=USPAT7176029 Google Patents]

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