Difference between revisions of "Part:BBa P1011:Design"
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<partinfo>BBa_P1010</partinfo>
 
<partinfo>BBa_P1010</partinfo>
  
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===References===
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==References==
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*"Positive-selection vectors using the F plasmid <i>ccdB</i> killer gene." Bernard P, Gabant P, Bahassi EM, Couturier M. 1994 Gene. Oct 11;148(1):71-4. [http://www.ncbi.nlm.nih.gov/pubmed/7926841 Pubmed]
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*US Patent Number 5,910,438 "Cloning and/or sequencing vector" Bernard P, Gabant P, University Libre de Bruxelles, 1999. [http://www.google.com/patents?vid=USPAT5910438 Google Patents]
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*US Patent Number 6,180,407 B1 "Cloning and/or sequencing vector" Bernard P, Gabant P, Universit Libre de Bruxelles, 2001. [http://www.google.com/patents?vid=USPAT6180407 Google Patents]
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*US Patent Number 7,176,029 B2 "Cloning and/or sequencing vector"  Bernard P, Gabant P, Universit Libre de Bruxelles, 2007. [http://www.google.com/patents?vid=USPAT7176029 Google Patents]
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*"Interactions of CcdB with DNA gyrase. Inactivation of Gyra, poisoning of the gyrase-DNA complex, and the antidote action of CcdA." Bahassi, et al. 1999 J. Biol. Chem. 274: 10936-10944 [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10196173&query_hl=2&itool=pubmed_ExternalLink Pubmed]
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*[[Help:Plasmids]]
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*[[Part:BBa_P1010]]

Revision as of 15:41, 10 March 2010

ccdB cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is derived from BBa_P1010.

The following changes have been made.

  1. ccdA- has been deleted since its presence is not necessary for ccdB's function as a toxin.
  2. Some sequence from the 5' end of BBa_P1010 has also been deleted in order to eliminate a BamHI site.
  3. Silent mutations have been introduced in order to eliminate useful restriction sites and rare codons.

Source

BBa_P1010


References

  • "Positive-selection vectors using the F plasmid ccdB killer gene." Bernard P, Gabant P, Bahassi EM, Couturier M. 1994 Gene. Oct 11;148(1):71-4. [http://www.ncbi.nlm.nih.gov/pubmed/7926841 Pubmed]
  • US Patent Number 5,910,438 "Cloning and/or sequencing vector" Bernard P, Gabant P, University Libre de Bruxelles, 1999. [http://www.google.com/patents?vid=USPAT5910438 Google Patents]
  • US Patent Number 6,180,407 B1 "Cloning and/or sequencing vector" Bernard P, Gabant P, Universit Libre de Bruxelles, 2001. [http://www.google.com/patents?vid=USPAT6180407 Google Patents]
  • US Patent Number 7,176,029 B2 "Cloning and/or sequencing vector" Bernard P, Gabant P, Universit Libre de Bruxelles, 2007. [http://www.google.com/patents?vid=USPAT7176029 Google Patents]
  • "Interactions of CcdB with DNA gyrase. Inactivation of Gyra, poisoning of the gyrase-DNA complex, and the antidote action of CcdA." Bahassi, et al. 1999 J. Biol. Chem. 274: 10936-10944 [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10196173&query_hl=2&itool=pubmed_ExternalLink Pubmed]
  • Help:Plasmids
  • Part:BBa_P1010