Difference between revisions of "Part:BBa K5443002"
 
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<partinfo>BBa_K5443002 short</partinfo>
 
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This enzyme from Saccharothrix espanaensis catalyzes the hydroxylation of 4-coumaric acid to make caffeic acid.  This is the second step of the vanillin biosynthesis pathway. This part builds on the work of earlier iGEM teams, who contributed C3H Parts as follows: BBa_I742105,  BBa_K238007,  BBa_K2906013.  
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<b>Coumaric acid 3-hydroxylase (C3H)</b> from <i>Saccharothrix espanaensis</i>, <B>Sam5</b>, functions as the second step in our vanillin biosynthesis pathway. It converts p-coumaric acid into caffeic acid via hydroxylation (Fig. 1).
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https://static.igem.wiki/teams/5443/c3h-reaction.jpg
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===Usage and Biology===
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This part builds on the work of earlier iGEM teams, who contributed C3H Parts as follows: [https://parts.igem.org/Part:BBa_I742105 BBa_I742105], [https://parts.igem.org/Part:BBa_K238007 BBa_K238007], and [https://parts.igem.org/Part:BBa_K2906013 BBa_K2906013].
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5443002 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<figure>
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<partinfo>BBa_K5443002 parameters</partinfo>
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    <img src="https://static.igem.wiki/teams/5443/c3h-reaction.jpg" style="width:600px;height:auto">
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    <figcaption>
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        <b>Figure 1. C3H-Sam5-catalysed conversion of p-coumaric acid to caffeic acid.</b><br>
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        <i>This reaction represents the second step in our vanillin synthesis pathway. The C3H enzyme Sam5 converts coumarate (p-coumaric acid) into caffeic acid.</i>
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Latest revision as of 13:47, 2 October 2024

Coumaric acid 3-hydroxylase (C3H)

Coumaric acid 3-hydroxylase (C3H) from Saccharothrix espanaensis, Sam5, functions as the second step in our vanillin biosynthesis pathway. It converts p-coumaric acid into caffeic acid via hydroxylation (Fig. 1).

This part builds on the work of earlier iGEM teams, who contributed C3H Parts as follows: BBa_I742105, BBa_K238007, and BBa_K2906013.


Figure 1. C3H-Sam5-catalysed conversion of p-coumaric acid to caffeic acid.
This reaction represents the second step in our vanillin synthesis pathway. The C3H enzyme Sam5 converts coumarate (p-coumaric acid) into caffeic acid.