Difference between revisions of "Part:BBa K5198107"

 
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<partinfo>BBa_K5198107 short</partinfo>
 
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nSEAP-NanoLuc is the NanoLuciferase (<a href="https://parts.igem.org/Part:BBa_K1734004">BBa_K1734004</a>
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) with native SEAP tag (<a href="https://parts.igem.org/Part:BBa_K5198010">BBa_K5198010</a>
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). Native SEAP tag is the native signal sequence of secreted embryonic alkaline phosphatase (SEAP). SEAP acts as a continuous quantitative reporter for gene expression by repeatedly assessing the activity of certain promoter/enhancer elements in many cell samples. SEAP is contained in exosomes and is secreted into the extracellular space. nSEAP tag sequence causes extacellular secretion of proteins. NanoLuciferase is a highly-stable enzyme that functions as a luminescent reporter. Its luminesecnt reaction is ATP-independent and very sensitive. The small size and extreme brightness of NanoLuc makes it an ideal fusion partner. When attached to the nSEAP tag, this nSEAP-NanoLuc signal peptide can be used in solubility studies to find the optimal secretion signal peptide for neoantigens in ATLAS.
  
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NanoLuciferase with native SEAP tag. This part was used in solubility studies to find the optimal secretion signal peptide for neoantigens in ATLAS.
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Revision as of 11:08, 2 October 2024


nSEAP-NanoLuc
nSEAP-NanoLuc is the NanoLuciferase (BBa_K1734004 ) with native SEAP tag (BBa_K5198010 ). Native SEAP tag is the native signal sequence of secreted embryonic alkaline phosphatase (SEAP). SEAP acts as a continuous quantitative reporter for gene expression by repeatedly assessing the activity of certain promoter/enhancer elements in many cell samples. SEAP is contained in exosomes and is secreted into the extracellular space. nSEAP tag sequence causes extacellular secretion of proteins. NanoLuciferase is a highly-stable enzyme that functions as a luminescent reporter. Its luminesecnt reaction is ATP-independent and very sensitive. The small size and extreme brightness of NanoLuc makes it an ideal fusion partner. When attached to the nSEAP tag, this nSEAP-NanoLuc signal peptide can be used in solubility studies to find the optimal secretion signal peptide for neoantigens in ATLAS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 571
    Illegal NgoMIV site found at 1540
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 901
    Illegal BsaI.rc site found at 1390