Difference between revisions of "Part:BBa K5246047"
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===Experimental characterization=== | ===Experimental characterization=== | ||
Latest revision as of 21:40, 1 October 2024
H.Baltica hfsE-hfsG-hfsH Part of the polysaccharide tetrad assembly system
Introduction
Vilnius-Lithuania iGEM 2024 project Synhesion aspires to create biodegradable and environmentally friendly adhesives. We were inspired by bacteria, which naturally produce adhesives made from polysaccharides. Two bacteria from aquatic environments - C. crescentus and H. baltica - harness 12 protein synthesis pathways to produce sugars, anchoring them to the surfaces. We aimed to transfer the polysaccharide synthesis pathway to industrially used E. coli bacteria to produce adhesives. Our team concomitantly focused on creating a novel E. coli strain for more efficient production of adhesives.
This is a part of the complete holdfast tetrad assembly system BBa_K5246049 used in Vilnius-Lithuania iGEM 2024 project "Synhesion" https://2024.igem.wiki/vilnius-lithuania/. This part can also be used separately for glycolipids consisting of glucose, mannosaminuronic acid, and N-acetyl-D-glucosamine synthesis, but this feature needs more detailed characterization.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2514
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2409
Illegal BamHI site found at 135 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 301
Illegal NgoMIV site found at 3061
Illegal AgeI site found at 1468 - 1000COMPATIBLE WITH RFC[1000]