Part:BBa_K5246049
H.Baltica hfsE-hfsG-hfsH-hfsJ-hfsL-hfsK Polysaccharide tetrad assembly system
Introduction
Vilnius-Lithuania iGEM 2024 project Synhesion aspires to create biodegradable and environmentally friendly adhesives. We were inspired by bacteria, which naturally produce adhesives made from polysaccharides. Two bacteria from aquatic environments - C. crescentus and H. baltica - harness 12 protein synthesis pathways to produce sugars, anchoring them to the surfaces. We aimed to transfer the polysaccharide synthesis pathway to industrially used E. coli bacteria to produce adhesives. Our team concomitantly focused on creating a novel E. coli strain for more efficient production of adhesives.
used in Vilnius-Lithuania iGEM 2024 project "Synhesion" https://2024.igem.wiki/vilnius-lithuania/
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 3763
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2514
Illegal PstI site found at 3763 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2409
Illegal BglII site found at 4197
Illegal BglII site found at 6366
Illegal BamHI site found at 135 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 3763
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 3763
Illegal NgoMIV site found at 301
Illegal NgoMIV site found at 3061
Illegal NgoMIV site found at 4518
Illegal AgeI site found at 1468
Illegal AgeI site found at 6416 - 1000COMPATIBLE WITH RFC[1000]
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