Difference between revisions of "Part:BBa K5117036"

Revision as of 15:57, 1 October 2024

PcotYZ-BsRBS-BsEglS-L2-CotY-B0014

This part serves as transcriptional unit composed of:

promoter PcotYZ of Bacillus subtilis (BBa_K5117021)
ribosome binding site of Bacillus subtilis (BBa_K5117000)
eglS gene of Bacillus subtilis without signal peptide encoding an endoglucanase (EC 3.2.1.4),

addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)₄ (BBa_K5117024)

Biosafety level: S1

Target organism: Bacillus subtilis

Main purpose of use: Immobilization of BsEglS on the spore crust of B. subtilis (spore surface display)

Application: Degradation of cellulose

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1696
Illegal AgeI site found at 726
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 <li>ribosome binding site of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117000">(BBa_K5117000)</a></html> </li>
-<li><i>eglS</i> gene of <i>Bacillus subtilis</i> without signal peptide encoding an endoglucanase (EC 3.2.1.4) </li>
+<li><i>eglS</i> gene of <i>Bacillus subtilis</i> without signal peptide encoding an endoglucanase (EC 3.2.1.4), </li>
 <div style="text-indent: 1px;">addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)<sub>4</sub> <html><a href="https://parts.igem.org/Part:BBa_K5117024">(BBa_K5117024)</a></html></div>