Difference between revisions of "Part:BBa K5117041"

Revision as of 15:47, 1 October 2024

PcotYZ-BsRBS-PpBglB-L1-CotY-B0014

This part serves as transcriptional unit composed of:

addition of a short flexible linker (L1) downstream of the coding sequence encoding the amino acids GA (BBa_K5117029)

Biosafety level: S1

Target organism: Bacillus subtilis

Main purpose of use: Immobilization of PpBglB on the spore crust of B. subtilis (spore surface display)

Application: Degradation of cellobiose

Sequence and Features

Assembly Compatibility:

@@ Line 6: / Line 6: @@
 <ul>
-<li>promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> (BBa_K5117021)</li>
+<li>promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117021">(BBa_K5117021)</a></html></li>
-<li>ribosome binding site of <i>Bacillus subtilis</i> (BBa_K5117000)</li>
+<li>ribosome binding site of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117000">(BBa_K5117000)</a></html></li>
-<li><i>bglB</i> gene of <i>Paenibacillus polymyxa</i> encoding a beta-glucosidase (EC 3.2.1.21)</li>
+<li><i>bglB</i> gene of <i>Paenibacillus polymyxa</i> encoding a β-glucosidase (EC 3.2.1.21)</li>
-addition of a short flexible linker (L1) downstream of the coding sequence encoding the amino acids GA (BBa_K5117029)</li>
+<div style="text-indent: 1px;">addition of a short flexible linker (L1) downstream of the coding sequence encoding the amino acids GA <html><a href="https://parts.igem.org/Part:BBa_K5117029">(BBa_K5117029)</a></html></div>
-<li><i>cotY</i> gene of <i>Bacillus subtilis</i> (BBa_K5117022)</li>
+<li><i>cotY</i> gene of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117022">(BBa_K5117022)</a></html></li>
-<li>bidirectional terminator B0014 (BBa_B0014)</li>
+<li>bidirectional terminator B0014 <html><a href="https://parts.igem.org/Part:BBa_B0014">(BBa_B0014)</a></html></li>
 </ul>