Difference between revisions of "Part:BBa K5322031:Design"

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Components from <a href="https://parts.igem.org/Part:BBa_J23119">BBa_J23119</a>, <a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>, <a href="https://parts.igem.org/Part:BBa_K5322034">BBa_K5322034</a>, <a href="https://parts.igem.org/Part:BBa_K731721">BBa_K731721</a>.
 
Components from <a href="https://parts.igem.org/Part:BBa_J23119">BBa_J23119</a>, <a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>, <a href="https://parts.igem.org/Part:BBa_K5322034">BBa_K5322034</a>, <a href="https://parts.igem.org/Part:BBa_K731721">BBa_K731721</a>.
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===References===
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==References==

Latest revision as of 15:47, 1 October 2024


Programmed Cell Death 1 Ligand 1 Functional Domain [Mus musculus] Expression System


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 377
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 519
    Illegal PstI site found at 377
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 277
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 377
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 377
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization was performed according to the E. coli codon preference table. PD-L1 was truncated, retaining the 1st to 150aa of the original PD-L1 290aa.

Source

Components from BBa_J23119, BBa_B0034, BBa_K5322034, BBa_K731721.

References