Difference between revisions of "Part:BBa K5117037"
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<ul>
 
<ul>
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<li>promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> (BBa_K5117021) </li>
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<li>promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117021">(BBa_K5117021) </a></html></li>
  
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<li>ribosome binding site of <i>Bacillus subtilis</i> (BBa_K5117000) </li>
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<li>ribosome binding site of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117000">(BBa_K5117000) </a></html></li>
  
 
<li><i>eglS</i> gene of <i>Bacillus subtilis</i> without signal peptide encoding an endoglucanase (EC 3.2.1.4) </li>
 
<li><i>eglS</i> gene of <i>Bacillus subtilis</i> without signal peptide encoding an endoglucanase (EC 3.2.1.4) </li>
  
−
<div style="text-indent: 1px;">addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG (BBa_K5117025) </div>
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<div style="text-indent: 1px;">addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG <html><a href="https://parts.igem.org/Part:BBa_K5117025">(BBa_K5117025)</a></html> </div>
  
−
<li><i>cotY</i> gene of <i>Bacillus subtilis</i> (BBa_K5117022) </li>
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<li><i>cotY</i> gene of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117022">(BBa_K5117022)</a></html> </li>
  
−
<li>bidirectional terminator B0014 (BBa_B0014) </li>
+
<li>bidirectional terminator B0014 <html><a href="https://parts.igem.org/Part:BBa_B0014">(BBa_B0014)</a></html> </li>
−
</u>
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</ul>
  
  

Revision as of 15:14, 1 October 2024


PcotYZ-BsRBS-BsEglS-L3-CotY-B0014

This part serves as transcriptional unit composed of:

  • promoter PcotYZ of Bacillus subtilis (BBa_K5117021)
  • ribosome binding site of Bacillus subtilis (BBa_K5117000)
  • eglS gene of Bacillus subtilis without signal peptide encoding an endoglucanase (EC 3.2.1.4)
    • addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG (BBa_K5117025)
  • cotY gene of Bacillus subtilis (BBa_K5117022)
  • bidirectional terminator B0014 (BBa_B0014)


Biosafety level: S1

Target organism: Bacillus subtilis

Main purpose of use: Immobilization of BsEglS on the spore crust of B. subtilis (spore surface display)

Application: Degradation of cellulose


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1669
    Illegal AgeI site found at 726
  • 1000
    COMPATIBLE WITH RFC[1000]