Difference between revisions of "Part:BBa K5117037"
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<ul> | <ul> | ||
− | <li>promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> (BBa_K5117021) </li> | + | <li>promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117021">(BBa_K5117021) </a></html></li> |
− | <li>ribosome binding site of <i>Bacillus subtilis</i> (BBa_K5117000) </li> | + | <li>ribosome binding site of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117000">(BBa_K5117000) </a></html></li> |
<li><i>eglS</i> gene of <i>Bacillus subtilis</i> without signal peptide encoding an endoglucanase (EC 3.2.1.4) </li> | <li><i>eglS</i> gene of <i>Bacillus subtilis</i> without signal peptide encoding an endoglucanase (EC 3.2.1.4) </li> | ||
− | <div style="text-indent: 1px;">addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG (BBa_K5117025) </div> | + | <div style="text-indent: 1px;">addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG <html><a href="https://parts.igem.org/Part:BBa_K5117025">(BBa_K5117025)</a></html> </div> |
− | <li><i>cotY</i> gene of <i>Bacillus subtilis</i> (BBa_K5117022) </li> | + | <li><i>cotY</i> gene of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117022">(BBa_K5117022)</a></html> </li> |
− | <li>bidirectional terminator B0014 (BBa_B0014) </li> | + | <li>bidirectional terminator B0014 <html><a href="https://parts.igem.org/Part:BBa_B0014">(BBa_B0014)</a></html> </li> |
− | </ | + | </ul> |
Revision as of 15:14, 1 October 2024
PcotYZ-BsRBS-BsEglS-L3-CotY-B0014
This part serves as transcriptional unit composed of:
- promoter PcotYZ of Bacillus subtilis (BBa_K5117021)
- ribosome binding site of Bacillus subtilis (BBa_K5117000)
- eglS gene of Bacillus subtilis without signal peptide encoding an endoglucanase (EC 3.2.1.4)
- cotY gene of Bacillus subtilis (BBa_K5117022)
- bidirectional terminator B0014 (BBa_B0014)
addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG (BBa_K5117025)
Biosafety level: S1
Target organism: Bacillus subtilis
Main purpose of use: Immobilization of BsEglS on the spore crust of B. subtilis (spore surface display)
Application: Degradation of cellulose
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1669
Illegal AgeI site found at 726 - 1000COMPATIBLE WITH RFC[1000]