Difference between revisions of "Part:BBa K5117036"
| Line 10: | Line 10: | ||
<li>ribosome binding site of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117000">(BBa_K5117000)</a></html> </li> | <li>ribosome binding site of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117000">(BBa_K5117000)</a></html> </li> | ||
| − | <li><i>eglS</ | + | <li><i>eglS</i> gene of <i>Bacillus subtilis</i> without signal peptide encoding an endoglucanase (EC 3.2.1.4) </li> |
<div style="text-indent: 1px;">addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 <html><a href="https://parts.igem.org/Part:BBa_K5117024">(BBa_K5117024)</a></html></div> | <div style="text-indent: 1px;">addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 <html><a href="https://parts.igem.org/Part:BBa_K5117024">(BBa_K5117024)</a></html></div> | ||
Revision as of 15:08, 1 October 2024
PcotYZ-BsRBS-BsEglS-L2-CotY-B0014
This part serves as transcriptional unit composed of:
- promoter PcotYZ of Bacillus subtilis (BBa_K5117021)
- ribosome binding site of Bacillus subtilis (BBa_K5117000)
- eglS gene of Bacillus subtilis without signal peptide encoding an endoglucanase (EC 3.2.1.4)
- cotY gene of Bacillus subtilis (BBa_K5117022)
- bidirectional terminator B0014 (BBa_B0014)
addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 (BBa_K5117024)
Biosafety level: S1
Target organism: Bacillus subtilis
Main purpose of use: Immobilization of BsEglS on the spore crust of B. subtilis (spore surface display)
Application: Degradation of cellulose
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1696
Illegal AgeI site found at 726 - 1000COMPATIBLE WITH RFC[1000]