Difference between revisions of "Part:BBa K5117036"
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This part serves as transcriptional unit composed of:
 
This part serves as transcriptional unit composed of:
  
−
- promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> (BBa_K5117021),  
+
<ul>
 +
<li>promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117021">(BBa_K5117021)</a></html>, </i>
  
−
- ribosome binding site of <i>Bacillus subtilis</i> (BBa_K5117000),
+
<li>ribosome binding site of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117000">(BBa_K5117000)</a></html> </i>
  
−
- <i>eglS</i> gene of <i>Bacillus subtilis</i> without signal peptide encoding an endoglucanase (EC 3.2.1.4),
+
<li><i>eglS</i> gene of <i>Bacillus subtilis</i> without signal peptide encoding an endoglucanase (EC 3.2.1.4), </i>
  
−
addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 (BBa_K5117024),
+
addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 <html><a href="https://parts.igem.org/Part:BBa_K5117024">(BBa_K5117024)</a></html> </i>
  
−
- <i>cotY</i> gene of <i>Bacillus subtilis</i> (BBa_K5117022),
+
<li><i>cotY</i> gene of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117022">(BBa_K5117022)</a></html> </i>
  
−
- bidirectional terminator B0014 (BBa_B0014).
+
<li>bidirectional terminator B0014 <html><a href="https://parts.igem.org/Part:BBa_B0014">(BBa_B0014)</a></html> </i>
 +
</ul>
  
 +
 +
<b>Biosafety level:</b> S1
 +
 +
<b>Target organism:</b> <i>Bacillus subtilis</i>
 +
 +
<b>Main purpose of use:</b> Immobilization of BsEglS on the spore crust of <i>B. subtilis</i> (spore surface display)
 +
 +
<b>Application:</b> Degradation of cellulose
  
  

Revision as of 14:58, 1 October 2024


PcotYZ-BsRBS-BsEglS-L2-CotY-B0014

This part serves as transcriptional unit composed of:

  • promoter PcotYZ of Bacillus subtilis (BBa_K5117021), </i>
  • ribosome binding site of Bacillus subtilis (BBa_K5117000) </i>
  • eglS gene of Bacillus subtilis without signal peptide encoding an endoglucanase (EC 3.2.1.4), </i> addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 (BBa_K5117024) </i>
  • cotY gene of Bacillus subtilis (BBa_K5117022) </i>
  • bidirectional terminator B0014 (BBa_B0014) </i> </ul> Biosafety level: S1 Target organism: Bacillus subtilis Main purpose of use: Immobilization of BsEglS on the spore crust of B. subtilis (spore surface display) Application: Degradation of cellulose Sequence and Features

    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      COMPATIBLE WITH RFC[12]
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      INCOMPATIBLE WITH RFC[25]
      Illegal NgoMIV site found at 1696
      Illegal AgeI site found at 726
    • 1000
      COMPATIBLE WITH RFC[1000]