Difference between revisions of "Part:BBa K191007"
 
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Presence of Tryptophan inhibits the expression of genes downstream of the regulator.  
 
Presence of Tryptophan inhibits the expression of genes downstream of the regulator.  
 
As the hybrid protein LovTAP ( <partinfo>BBa_K191006</partinfo> ) contains the corresponding regulatory domain, LovTAP can also bind to the regulator and and interferes with downstream gene's expression.
 
As the hybrid protein LovTAP ( <partinfo>BBa_K191006</partinfo> ) contains the corresponding regulatory domain, LovTAP can also bind to the regulator and and interferes with downstream gene's expression.
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<br><br>
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===  Contribution from LCG-Global 2024 ===
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This year, our project focused on the development of a standardized biomarker detection system. This part serves as the sensitive promoter in the R module of our tryptophan detection system:
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<html>
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  <div style="display: flex; justify-content: center; align-items: center;">
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    <img src="https://static.igem.wiki/teams/5460/part-registry/bba-k191007.jpg" alt="图1" style="width: 300px; margin-right: 10px;">
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</div>
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</html>
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By combining it with our A module, we validated the design of our standardized system and achieved a response to different concentrations of tryptophan:
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<html>
 +
<div style="display: flex; justify-content: center; align-items: center;">
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    <img src="https://static.igem.wiki/teams/5460/engineering/bj1-eng-7.jpg" alt="图2" style="width: 280px; margin-right: 10px;">
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</div>
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</html>
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Additionally, we performed a Hill equation fit for this system:
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<html>
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<div style="display: flex; justify-content: center; align-items: center;">
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    <img src="https://static.igem.wiki/teams/5460/engineering/bj1-eng-8.jpg" alt="图3" style="width: 250px; margin-right: 10px;">
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</div>
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</html>
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Our work provides more insights into the response intensity of this part under varying tryptophan concentrations, offering valuable references for future iGEM teams.
  
 
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Latest revision as of 05:49, 1 October 2024

Trp promoter

Promoter of Tryptophan operon ( Tryptophan operon is an operon that codes for the components of tryptophan's synthesis)

Usage and Biology

Presence of Tryptophan inhibits the expression of genes downstream of the regulator. As the hybrid protein LovTAP ( BBa_K191006 ) contains the corresponding regulatory domain, LovTAP can also bind to the regulator and and interferes with downstream gene's expression.

Contribution from LCG-Global 2024

This year, our project focused on the development of a standardized biomarker detection system. This part serves as the sensitive promoter in the R module of our tryptophan detection system:

图1
By combining it with our A module, we validated the design of our standardized system and achieved a response to different concentrations of tryptophan:
图2
Additionally, we performed a Hill equation fit for this system:
图3
Our work provides more insights into the response intensity of this part under varying tryptophan concentrations, offering valuable references for future iGEM teams.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 1000
    COMPATIBLE WITH RFC[1000]