Difference between revisions of "Part:BBa K292003"
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<partinfo>BBa_K292003 short</partinfo> | <partinfo>BBa_K292003 short</partinfo> | ||
| − | This part contains a kanamycin resistance cassette and a double terminator (BBa_P1003 + BBa_B0014). This allows the kanamycin cassette to be functional. <br> | + | This part contains a kanamycin resistance cassette and a double terminator ([[Part:BBa_P1003|BBa_P1003]] + [[Part:BBa_B0014|BBa_B0014]]). This allows the kanamycin cassette to be functional. <br> |
This part is completed by BBa_K292004 [https://parts.igem.org/Part:BBa_K292004] to finish the transcription before the resistance cassette. <br> | This part is completed by BBa_K292004 [https://parts.igem.org/Part:BBa_K292004] to finish the transcription before the resistance cassette. <br> | ||
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Theres is no special functional parameters. | Theres is no special functional parameters. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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| + | <partinfo>BBa_K292003 SequenceAndFeatures</partinfo> | ||
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<partinfo>BBa_K292003 parameters</partinfo> | <partinfo>BBa_K292003 parameters</partinfo> | ||
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Revision as of 23:23, 21 October 2009
Kanamycin resistance cassette and a double terminator
This part contains a kanamycin resistance cassette and a double terminator (BBa_P1003 + BBa_B0014). This allows the kanamycin cassette to be functional.
This part is completed by BBa_K292004 [1] to finish the transcription before the resistance cassette.
Usage and Biology
This BioBrick gives kanamycin resistance.
The cassette also can be used to design a recombinant vector by gene insertion in a non lytic bacteriophage as the Lambda phage.
In fact it allows us to know if a bacteriophage is modified or not and if the inserted gene is expressed or not. When the virus is modified the kanamycin cassette induces a kanamycin resistance, so the bacteria become resistance, and when the virus is not modified bacteria are killed by kanamycin.
Functional Parameters
Theres is no special functional parameters.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| biology | T7TE-LuxIA |
| direction | Bidirectional |
| forward_efficiency | 0.604[CC]/0.96[JK] |
| o_h | -NA- |
| protein | kanamycin resistance protein |
| rbs | -NA- |
| reversed_version | 2145 |
| reverse_efficiency | 0.86[JK] |
| tag | None |