Difference between revisions of "Part:BBa K292002"
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| − | This part contains the pLac promoter and a ribosome binding site (BBa_R0010 + BBa_B0030). The Lac promoter is constitutively activated, but it can be repressed by the Lac I repressor from E.coli. <br> | + | This part contains the pLac promoter and a ribosome binding site ([[Part:BBa_R0010|BBa_R0010]] + [[Part:BBa_B0030|BBa_B0030]]). The Lac promoter is constitutively activated, but it can be repressed by the Lac I repressor from E.coli. <br> |
=== Usage in and Biology === | === Usage in and Biology === | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
| − | This BioBrick must be use with the LacI from <i>E.coli</i> BBa_C0012 [https://parts.igem.org/Part:BBa_C0012]. <br> | + | This BioBrick must be use with the LacI from <i>E.coli</i> [[Part:BBa_C0012|BBa_C0012]] [https://parts.igem.org/Part:BBa_C0012]. <br> |
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<partinfo>BBa_K292002 parameters</partinfo> | <partinfo>BBa_K292002 parameters</partinfo> | ||
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Revision as of 23:17, 21 October 2009
pLac (LacI regulated) + Strong RBS
This part contains the pLac promoter and a ribosome binding site (BBa_R0010 + BBa_B0030). The Lac promoter is constitutively activated, but it can be repressed by the Lac I repressor from E.coli.
Usage in and Biology
This promoter is really useful to control a gene expression by inactivating it with LacI repressor. When LacI is absent the gene should be expressed, and in the presence of LacI, pLac is inhibited. So this part is really suitable for gene expression control by inactivation of pLac with LacI.
We can use this part to control a failed feedback mechanism which induces the Lambda phage lytic cycle.
In E.Coli, pLac is a part of the lactose operon. LacI is constantly expressed and represses the pLac. In the presence of Lactose, LacI is inhibited and pLac is active and allows the transcription of beta-galactosidase. Lactose can be substituted by IPTG (Isopropyl β-D-1-thiogalactopyranoside).
This part already contains a strong RBS to begin directly a gene transcription.
Functional Parameters
This BioBrick must be use with the LacI from E.coli BBa_C0012 [1].
| biology | -NA- |
| control | IPTG, lacI |
| direction | Forward |
| efficiency | 0.6 |
| negative_regulators | 1 |
| o_h | -NA- |
| o_l | -NA- |
| positive_regulators | -NA- |
| rbs | -NA- |