Difference between revisions of "Part:BBa K292001"
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This part contains a double terminator and a constitutive promoter family member (prokaryote)and a strong RBS ([[Part:BBa_B0014|BBa_B0014]] + [[Part:BBa_J23100|BBa_J23100]] + [[Part:BBa_B0030|BBa_B0030]]. It comes from [[Part:BBa_J23100|BBa_J23100]]. <br>
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This part contains a double terminator and a constitutive promoter family member (prokaryote)and a strong RBS ([[Part:BBa_B0014|BBa_B0014]] + [[Part:BBa_J23100|BBa_J23100]] + [[Part:BBa_B0030|BBa_B0030]]). It comes from [[Part:BBa_J23100|BBa_J23100]]. <br>
  
 
== Usage and Biology ==
 
== Usage and Biology ==

Revision as of 23:14, 21 October 2009

Double terminator + Constitutive promoter + Strong RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 110
    Illegal NheI site found at 133
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


This part contains a double terminator and a constitutive promoter family member (prokaryote)and a strong RBS (BBa_B0014 + BBa_J23100 + BBa_B0030). It comes from BBa_J23100.

Usage and Biology

It is a simple promoter in the goals to begin gene transcription with terminator before it. The BioBrick can be usefull if we want to add a coding gene in front of the BioBrick and start a new transcription.

Sources

BBa_B0014 : Designed by Reshma Shetty Group: Registry (2003-07-16).
BBa_J23100 : Designed by John Anderson Group: iGEM2006_Berkeley (2006-08-04).
Isolated from a library of promoters.