Difference between revisions of "Part:BBa K292003"

 
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<partinfo>BBa_K292003 short</partinfo>
 
<partinfo>BBa_K292003 short</partinfo>
  
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This part contains a kanamycin resistance cassette and a double terminator (BBa_P1003 + BBa_B0014). This allows the kanamycin cassette to be functional.  
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This part contains a kanamycin resistance cassette and a double terminator (BBa_P1003 + BBa_B0014). This allows the kanamycin cassette to be functional. <br>
  
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The cassette can be used to design a recombinant vector by gene insertion in a non lytic bacteriophage as the Lambda phage.  
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This part is completed by BBa_K292004 [https://parts.igem.org/Part:BBa_K292004] to finish the transcription before the resistance cassette. <br>
  
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In fact it allows us to know if a bacteriophage is modified or not and if the inserted gene is expressed or not. When the virus is modified the kanamycin cassette induces a kanamycin resistance, so the bacteria become resistance, and when the virus is not modified bacteria are killed by kanamycin.
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===Usage and Biology===
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This part is completed by B0014 + P1003 + B0014 to finish the transcription before the resistance cassette.
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This BioBrick gives kanamycin resistance.
  
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The cassette also can be used to design a recombinant vector by gene insertion in a non lytic bacteriophage as the Lambda phage. <br>
  
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<!-- Add more about the biology of this part here
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In fact it allows us to know if a bacteriophage is modified or not and if the inserted gene is expressed or not. When the virus is modified the kanamycin cassette induces a kanamycin resistance, so the bacteria become resistance, and when the virus is not modified bacteria are killed by kanamycin. <br>
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===Usage and Biology===
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=== Functional Parameters ===
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Theres is no special functional parameters.
  
 
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Revision as of 20:50, 21 October 2009

Kanamycin resistance cassette and a double terminator

This part contains a kanamycin resistance cassette and a double terminator (BBa_P1003 + BBa_B0014). This allows the kanamycin cassette to be functional.

This part is completed by BBa_K292004 [1] to finish the transcription before the resistance cassette.

Usage and Biology

This BioBrick gives kanamycin resistance.

The cassette also can be used to design a recombinant vector by gene insertion in a non lytic bacteriophage as the Lambda phage.

In fact it allows us to know if a bacteriophage is modified or not and if the inserted gene is expressed or not. When the virus is modified the kanamycin cassette induces a kanamycin resistance, so the bacteria become resistance, and when the virus is not modified bacteria are killed by kanamycin.

Functional Parameters

Theres is no special functional parameters.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]