Difference between revisions of "Part:BBa K5349005:Design"
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===Source=== | ===Source=== | ||
| − | UL23 : | + | *UL23 : Refer to K5349000 https://parts.igem.org/Part:BBa_K5349000:Design |
| − | + | *sfGFP : Refer to K2553008 https://parts.igem.org/Part:BBa_K2553008:Design | |
| − | + | ||
===References=== | ===References=== | ||
Revision as of 06:37, 26 September 2024
UL23-sfGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1611
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 879
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For cloning, overlap sequences were designed for each fragment, and the assembly was performed using NEBuilder. Each fragment was synthesized using Twist Bioscience's DNA synthesis service
Source
- UL23 : Refer to K5349000 https://parts.igem.org/Part:BBa_K5349000:Design
- sfGFP : Refer to K2553008 https://parts.igem.org/Part:BBa_K2553008:Design