Difference between revisions of "Part:BBa K5205004"

 
 
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This part consists of a cell membrane display system, Non-OmpA [https://parts.igem.org/Part:BBa_K5205003 BBa_K5205003], fused with a rice metallothionein, OsMTI-1b (also known as OsMT1, [https://parts.igem.org/Part:BBa_K5205000 BBa_K5205000]).
 
This part consists of a cell membrane display system, Non-OmpA [https://parts.igem.org/Part:BBa_K5205003 BBa_K5205003], fused with a rice metallothionein, OsMTI-1b (also known as OsMT1, [https://parts.igem.org/Part:BBa_K5205000 BBa_K5205000]).
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K5205004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5205004 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K5205004 parameters</partinfo>
 
<partinfo>BBa_K5205004 parameters</partinfo>
 
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===Usage and Biology===
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The fusion of Non-OmpA with OsMTI-1b enables the metallothionein to be displayed on the E. coli cell surface, allowing the fusion OsMT1 to effectively bind heavy metal ions in the environment. We expressed this fusion protein in E. coli BL21 to turn it into a heavy metal remover.
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<center><img src = "https://static.igem.wiki/teams/5205/parts/04-1.png" style = "width:400px"></center>
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<figcaption><center>Figure 1. SDS-PAGE of total protein of engineered E. coli BL21 expressing Non-OmpA-OsMTI-1b.  </center></figcaption>
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Latest revision as of 02:57, 24 September 2024


Non-OmpA-OsMTI-1b

This part consists of a cell membrane display system, Non-OmpA BBa_K5205003, fused with a rice metallothionein, OsMTI-1b (also known as OsMT1, BBa_K5205000).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

The fusion of Non-OmpA with OsMTI-1b enables the metallothionein to be displayed on the E. coli cell surface, allowing the fusion OsMT1 to effectively bind heavy metal ions in the environment. We expressed this fusion protein in E. coli BL21 to turn it into a heavy metal remover.

Figure 1. SDS-PAGE of total protein of engineered E. coli BL21 expressing Non-OmpA-OsMTI-1b.