Difference between revisions of "Part:BBa K191009"

 
 
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<partinfo>BBa_K191009 short</partinfo>
 
<partinfo>BBa_K191009 short</partinfo>
  
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See part BBa_K191005 for information on fused protein LOVTAP. This part is a mutated version of LOVTAP: amino acid #427 ILE is mutated into PHE  
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Mutated version of <partinfo>BBa_K191006</partinfo> : the amino acid #427 ILE was mutated into PHE.
  
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===Usage and Biology===
 
===Usage and Biology===
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The reasons and choix of this mutation are given by the results of modeling, under [http://2009.igem.org/wiki/index.php?title=Team:EPF-Lausanne/Results/Mutations Mutations]' section.
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The mutated version of LovTAP should react faster and the conformational change should be more stable ( the protein stays in the active form for longer).
  
 
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Latest revision as of 14:37, 21 October 2009

LOVTAP (ILE427PHE)

Mutated version of BBa_K191006 : the amino acid #427 ILE was mutated into PHE.

Usage and Biology

The reasons and choix of this mutation are given by the results of modeling, under [http://2009.igem.org/wiki/index.php?title=Team:EPF-Lausanne/Results/Mutations Mutations]' section.

The mutated version of LovTAP should react faster and the conformational change should be more stable ( the protein stays in the active form for longer).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 535