Difference between revisions of "Part:BBa K5317001"
Line 3: Line 3:
 
<partinfo>BBa_K5317001 short</partinfo>
 
<partinfo>BBa_K5317001 short</partinfo>
  
−
 
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
The reporter gene mRuby was engineered in 2009 as a variant of the red fluorescent protein eqFP611 by Kredel ''et al.'' and developed further to mRuby2 by Lam and colleagues in 2012. Its excitation maxima is at 559 nm and emission maxima at 600 nm. Due to the large Stokes shift it is well suited for imaging purposes since it reduces spectral overlap when multiple reporters are used as in our case in co-transfection experiments together with EGFP ((<span class="plainlinks">[https://parts.igem.org/Part:BBa_K3338006 K3338006]</span>)).  Similar to the findings of Kredel and colleagues (2009) is mRuby2 characterized by a higher resistance to photobleaching compared to the predecessor eqFP611 allowing long-term imaging experiments (Lam ''et al.'' 2012).
  
 
<!-- -->
 
<!-- -->
Line 11: Line 11:
 
<partinfo>BBa_K5317001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5317001 SequenceAndFeatures</partinfo>
  
 +
=References=
 +
 +
Kredel, S., Oswald, F., Nienhaus, K., Deuschle, K., Röcker, C., Wolff, M., Heilker, R., Nienhaus, G. U., & Wiedenmann, J. (2009). mRuby, a bright monomeric red fluorescent protein for labeling of subcellular structures. PloS one, 4(2), e4391. https://doi.org/10.1371/journal.pone.0004391
 +
 +
Lam, A. J., St-Pierre, F., Gong, Y., Marshall, J. D., Cranfill, P. J., Baird, M. A., McKeown, M. R., Wiedenmann, J., Davidson, M. W., Schnitzer, M. J., Tsien, R. Y., & Lin, M. Z. (2012). Improving FRET dynamic range with bright green and red fluorescent proteins. Nature methods, 9(10), 1005–1012. https://doi.org/10.1038/nmeth.2171
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 09:37, 16 September 2024


mRuby2

Usage and Biology

The reporter gene mRuby was engineered in 2009 as a variant of the red fluorescent protein eqFP611 by Kredel et al. and developed further to mRuby2 by Lam and colleagues in 2012. Its excitation maxima is at 559 nm and emission maxima at 600 nm. Due to the large Stokes shift it is well suited for imaging purposes since it reduces spectral overlap when multiple reporters are used as in our case in co-transfection experiments together with EGFP ((K3338006)). Similar to the findings of Kredel and colleagues (2009) is mRuby2 characterized by a higher resistance to photobleaching compared to the predecessor eqFP611 allowing long-term imaging experiments (Lam et al. 2012).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16

References

Kredel, S., Oswald, F., Nienhaus, K., Deuschle, K., Röcker, C., Wolff, M., Heilker, R., Nienhaus, G. U., & Wiedenmann, J. (2009). mRuby, a bright monomeric red fluorescent protein for labeling of subcellular structures. PloS one, 4(2), e4391. https://doi.org/10.1371/journal.pone.0004391

Lam, A. J., St-Pierre, F., Gong, Y., Marshall, J. D., Cranfill, P. J., Baird, M. A., McKeown, M. R., Wiedenmann, J., Davidson, M. W., Schnitzer, M. J., Tsien, R. Y., & Lin, M. Z. (2012). Improving FRET dynamic range with bright green and red fluorescent proteins. Nature methods, 9(10), 1005–1012. https://doi.org/10.1038/nmeth.2171