Difference between revisions of "Part:BBa K4601254"

 
 
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<partinfo>BBa_K4601254 short</partinfo>
 
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This part is an expression cassette of the CmR gene coding for the chloramphenicol ''O''-acetyltransferase enzyme ([[Part:BBa_K4601051|BBa_K4601051]]) under the control of the [[Part:BBa_J23110|BBa_J23110]] constitutive promoter.
  
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===Usage and Biology===
 
===Usage and Biology===
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This part expresses constitutively the CmR gene coding for the chloramphenicol ''O''-acetyltransferase enzyme ([[Part:BBa_K4601051|BBa_K4601051]]). It can be used as a positive control in various experiments.
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''E. coli'' cells carrying it are resistant to the chloramphenicol antibiotic.
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Latest revision as of 13:55, 12 October 2023


CmR expression cassette under the control of the J23110 promoter

This part is an expression cassette of the CmR gene coding for the chloramphenicol O-acetyltransferase enzyme (BBa_K4601051) under the control of the BBa_J23110 constitutive promoter.

Usage and Biology

This part expresses constitutively the CmR gene coding for the chloramphenicol O-acetyltransferase enzyme (BBa_K4601051). It can be used as a positive control in various experiments.

E. coli cells carrying it are resistant to the chloramphenicol antibiotic.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]