Difference between revisions of "Part:BBa K4601244"

 
 
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<partinfo>BBa_K4601244 short</partinfo>
 
<partinfo>BBa_K4601244 short</partinfo>
  
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This part is an expression cassette of the AmpR, an ''E. coli'' gene coding for a β-lactamase enzyme ([[Part:BBa_K4601041|BBa_K4601041]]) under the control of the [[Part:BBa_J23110|BBa_J23110]] constitutive promoter.
  
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===Usage and Biology===
 
===Usage and Biology===
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This part expresses constitutively the AmpR, an ''E. coli'' gene coding for a β-lactamase enzyme ([[Part:BBa_K4601041|BBa_K4601041]]). It can be used as a positive control in various experiments.
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''E. coli'' cells carrying it are resistant to β-lactam antibiotics like ampicillin or carbenicillin.
  
 
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Latest revision as of 13:54, 12 October 2023


AmpR expression cassette under the control of the J23110 promoter

This part is an expression cassette of the AmpR, an E. coli gene coding for a β-lactamase enzyme (BBa_K4601041) under the control of the BBa_J23110 constitutive promoter.

Usage and Biology

This part expresses constitutively the AmpR, an E. coli gene coding for a β-lactamase enzyme (BBa_K4601041). It can be used as a positive control in various experiments.

E. coli cells carrying it are resistant to β-lactam antibiotics like ampicillin or carbenicillin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]