Difference between revisions of "Part:BBa K243010"
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===Purification=== | ===Purification=== | ||
We achieved to purify one part of our universal endonuclease by a Histidin column purification and proved it with a following Western Blot. | We achieved to purify one part of our universal endonuclease by a Histidin column purification and proved it with a following Western Blot. | ||
| − | [[Image:WB09.10.02jpg.jpg]] | + | [[Image:WB09.10.02jpg.jpg]]<br> |
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| + | Western Blot: His-Flu_a-Split_Fok_i in pEx;<br> lanes: NEB prestained marker broad range, | ||
| + | <br> pool of elution fractions 2-5, empty lane, 3 positive controls | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 17:39, 20 October 2009
His-FluA-Split Linker-Fok_i
These part units some of our parts. The combination of a Histidin-Tag, a Anticalin-Tag and the linked protein domain Fok_a, makes it possible to purifier and detect the function of the protein. The fluoresceinA tag allows the measurement by quenching and the coupling to an flurescein linked oligo.
Purification
We achieved to purify one part of our universal endonuclease by a Histidin column purification and proved it with a following Western Blot.
Western Blot: His-Flu_a-Split_Fok_i in pEx;
lanes: NEB prestained marker broad range,
pool of elution fractions 2-5, empty lane, 3 positive controls
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 272
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]