Difference between revisions of "Part:BBa K200029"

Revision as of 14:40, 20 October 2009

pLacI+RBS+RcsB+TT

This is the functional transcriptional unit which allows expression of the RcsB receiver protein and positive regulation of colanic acid production.

The LacI promoter is normally inhibited by the Lac inhibitor, preventing any downstream translation. When IPTG is added, it binds to the Lac inhibitor, preventing inhibition of the promoter, allowing downstream expression of RcsB.

Usage and Biology

This composite part, comprising of subparts BBa_R0011, BBa_B0034, BBa_K200000 and BBa_B0015, was used in [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project by Imperial iGEM 2009.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 22:30, 18 October 2009 (view source) Royah (Talk \| contribs) ← Older edit		Revision as of 14:40, 20 October 2009 (view source) Royah (Talk \| contribs) Newer edit →
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	This is the functional transcriptional unit which allows expression of the RcsB receiver protein and positive regulation of colanic acid production.		This is the functional transcriptional unit which allows expression of the RcsB receiver protein and positive regulation of colanic acid production.
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		+	The LacI promoter is normally inhibited by the Lac inhibitor, preventing any downstream translation. When IPTG is added, it binds to the Lac inhibitor, preventing inhibition of the promoter, allowing downstream expression of RcsB.