Difference between revisions of "Part:BBa K200021"
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<partinfo>BBa_K200021 short</partinfo> | <partinfo>BBa_K200021 short</partinfo> | ||
| − | This BioBrick comprises a ligation of the registry parts for the promoter LacI+PL () and the RBS ([[Part:BBa_B0034 |BBa_B0034]]). | + | This BioBrick comprises a ligation of the registry parts for the promoter LacI+PL ([[Part:BBa_R0011 |BBa_R0011]]) and the RBS ([[Part:BBa_B0034 |BBa_B0034]]). |
| − | The LacI promoter is IPTG | + | The LacI promoter is normally inhibited by the Lac inhibitor, preventing any downstream translation. When IPTG is added, it binds to the Lac inhibitor, preventing inhibition of the promoter, allowing downstream translation. |
This promoter has been ligated to an RBS as an intermediate step of ligations. By adding a coding region followed by terminator after this part, one would create a glucose sensitive functional unit. | This promoter has been ligated to an RBS as an intermediate step of ligations. By adding a coding region followed by terminator after this part, one would create a glucose sensitive functional unit. | ||
Revision as of 22:27, 18 October 2009
pLacI+RBS
This BioBrick comprises a ligation of the registry parts for the promoter LacI+PL (BBa_R0011) and the RBS (BBa_B0034).
The LacI promoter is normally inhibited by the Lac inhibitor, preventing any downstream translation. When IPTG is added, it binds to the Lac inhibitor, preventing inhibition of the promoter, allowing downstream translation.
This promoter has been ligated to an RBS as an intermediate step of ligations. By adding a coding region followed by terminator after this part, one would create a glucose sensitive functional unit.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]