Difference between revisions of "Part:BBa K4813000"
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− | + | ===Usage and Biology=== | |
Comparing the colouration of optimized dTomato <partinfo>BBa_K4813000</partinfo> and tdTomato <partinfo>BBa_K4813001</partinfo></b> | Comparing the colouration of optimized dTomato <partinfo>BBa_K4813000</partinfo> and tdTomato <partinfo>BBa_K4813001</partinfo></b> |
Revision as of 09:42, 7 October 2023
dTomato fluoresence protein Codon Optimized for E coli
This part encodes a protein called dTomato, which is a red fluorescence protein. It forms a dimer and has a molecular weight of around 27.0 kDa. This protein originates from a species called Discosoma [1].
To enhance its performance for our project, we used IDT codon optimization tools to optimize the sequence. We incorporated this optimized sequence into two composite parts. One part served as a reporter gene for detecting formaldehyde with pFrmR formaldehyde sensing promoter (BBa_K4813003), while the other part (BBa_K4813005) acted as a positive control, where the expression of dTomato was driven by a strong constitutive promoter (BBa_J23100).
Reference
[1] dTomato at fpbase. FPbase. Accessed 20 June 2023 https://www.fpbase.org/protein/dtomato/
[2] tdTomato at fpbase. FPbase. Accessed 20 June 2023 https://www.fpbase.org/protein/tdtomato/
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]