Difference between revisions of "Part:BBa K4813000"
Line 6: Line 6:
  
 
To enhance its performance for our project, we used IDT codon optimization tools to optimize the sequence. We incorporated this optimized sequence into two composite parts. One part served as a reporter gene for detecting formaldehyde with ''pFrmR'' formaldehyde sensing promoter (<partinfo>BBa_K4813003</partinfo>), while the other part (<partinfo>BBa_K4813005</partinfo>) acted as a positive control, where the expression of ''dTomato'' was driven by a strong constitutive promoter (<partinfo>BBa_J23100</partinfo>).  
 
To enhance its performance for our project, we used IDT codon optimization tools to optimize the sequence. We incorporated this optimized sequence into two composite parts. One part served as a reporter gene for detecting formaldehyde with ''pFrmR'' formaldehyde sensing promoter (<partinfo>BBa_K4813003</partinfo>), while the other part (<partinfo>BBa_K4813005</partinfo>) acted as a positive control, where the expression of ''dTomato'' was driven by a strong constitutive promoter (<partinfo>BBa_J23100</partinfo>).  
 +
 +
Reference
 +
[1] dTomato at fpbase. FPbase. Accessed 20 June 2023 https://www.fpbase.org/protein/dtomato/
 +
[2] tdTomato at fpbase. FPbase. Accessed 20 June 2023 https://www.fpbase.org/protein/tdtomato/
 +
 +
<!-- Add more about the biology of this part here
  
 
===Comparing the colouration of optimized dTomato <partinfo>BBa_K4813000</partinfo> and tdTomato <partinfo>BBa_K4813001</partinfo>===
 
===Comparing the colouration of optimized dTomato <partinfo>BBa_K4813000</partinfo> and tdTomato <partinfo>BBa_K4813001</partinfo>===
Line 18: Line 24:
 
Consequently, we opted to use dTomato for the final assay, as it proved to be more effective in detecting the presence of formaldehyde.
 
Consequently, we opted to use dTomato for the final assay, as it proved to be more effective in detecting the presence of formaldehyde.
  
−
===Reference===
 
−
[1] dTomato at fpbase. FPbase. Accessed 20 June 2023 https://www.fpbase.org/protein/dtomato/
 
  
−
[2] tdTomato at fpbase. FPbase. Accessed 20 June 2023 https://www.fpbase.org/protein/tdtomato/
 
−
 
−
<!-- Add more about the biology of this part here
 
  
  

Revision as of 09:33, 7 October 2023


dTomato fluoresence protein Codon Optimized for E coli

This part encodes a protein called dTomato, which is a red fluorescence protein. It forms a dimer and has a molecular weight of around 27.0 kDa. This protein originates from a species called Discosoma [1].

To enhance its performance for our project, we used IDT codon optimization tools to optimize the sequence. We incorporated this optimized sequence into two composite parts. One part served as a reporter gene for detecting formaldehyde with pFrmR formaldehyde sensing promoter (BBa_K4813003), while the other part (BBa_K4813005) acted as a positive control, where the expression of dTomato was driven by a strong constitutive promoter (BBa_J23100).

Reference [1] dTomato at fpbase. FPbase. Accessed 20 June 2023 https://www.fpbase.org/protein/dtomato/ [2] tdTomato at fpbase. FPbase. Accessed 20 June 2023 https://www.fpbase.org/protein/tdtomato/

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]