Difference between revisions of "Part:BBa K4390061"
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'''This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard [[Help:Standards/Assembly/Type_IIS|which is also accepted by iGEM.]]'''
 
'''This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard [[Help:Standards/Assembly/Type_IIS|which is also accepted by iGEM.]]'''
 +
 +
'''This is a level 1 part formed by assembly of the following level 0 parts:'''
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{| class="wikitable" style="margin:auto"
 +
|-
 +
| Promoter || [[part:BBa_J23100|J23100]]
 +
|-
 +
| RBS || [[part:BBa_B0034|B0034]]
 +
|-
 +
| N part || [[part:BBa_K4390016|K4390016]]
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|-
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| O part || [[part:BBa_K4390017|K4390017]]
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|-
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| C part || [[part:BBa_K4390009|K4390009]]
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|-
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| Terminator || [[part:BBa_K4390001|K4390001]]
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|}
  
 
==Usage and Biology==
 
==Usage and Biology==

Revision as of 23:43, 13 October 2022


Car9-tagged M. edulis metallothionein

This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.

This is a level 1 part formed by assembly of the following level 0 parts:

Promoter J23100
RBS B0034
N part K4390016
O part K4390017
C part K4390009
Terminator K4390001

Usage and Biology

Metallothionein (MT) is a small protein (around 6-7 kDa) which is rich in cysteine. These thiol group in cysteines provide ability to chelate almost all heavy metal ions including Cd2+, Hg2+, Pb2+ and As3+, but had been shown that has higher binding affinity with Hg2+ (Manceau, A. et al., 2019). The ability of chelating heavy metals provides the metal tolerance for its hosts. This MT sequence was obtained from Mytilus edulis, a blue mussel which originally live in aqueous environment (MACKAY E. A. et al.,1993).

This is one of the three types of silica-binding tags we explored for immobilising metallothioneins to silica beads. Car9 was also a silica-tag part used by the 2021 Edinburgh OG Team.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

MACKAY, E. A. et al. (1993) Complete amino acid sequences of five dimeric and four monomeric forms of metallothionein from the edible mussel Mytilus edulis. European journal of biochemistry. 218 (1), 183–194.

Manceau, A. et al. (2019) Mercury(II) Binding to Metallothionein in Mytilus edulis revealed by High Energy‐Resolution XANES Spectroscopy. Chemistry : a European journal. 25 (4), 997–1009.