Difference between revisions of "Part:BBa K4162007"

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[[File:T--Fudan--logo-Rester-rectangle.jpg|100px|right|2022 Fudan]]
 
[[File:T--Fudan--logo-Rester-rectangle.jpg|100px|right|2022 Fudan]]
  
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MS2 Coat Protein (MCP) is a capsid protein that self-assembles to a capsid. The protein can bind to the MS2-stemloop and acts as an adapter for RNA binding to the interest protein.<ref>Guo, Haotian et al. “Spatial engineering of E. coli with addressable phase-separated RNAs.” Cell vol. 185,20 (2022): 3823-3837.e23. doi:10.1016/j.cell.2022.09.016</ref>
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MS2 Coat Protein (MCP) is a capsid protein that self-assembles to a capsid. The protein can bind to the MS2-stemloop and acts as an adapter for RNA binding to the interest protein.<ref>Guo, Haotian et al. “Spatial engineering of ''E. coli'' with addressable phase-separated RNAs.” Cell vol. 185,20 (2022): 3823-3837.e23. doi:10.1016/j.cell.2022.09.016</ref>
  
 
[https://parts.igem.org/Part:BBa_K4162006 Dimer] MCP was used in [https://www.biorxiv.org/content/10.1101/2020.07.02.182527v2 TEARS].
 
[https://parts.igem.org/Part:BBa_K4162006 Dimer] MCP was used in [https://www.biorxiv.org/content/10.1101/2020.07.02.182527v2 TEARS].

Latest revision as of 16:15, 13 October 2022


MCP

Introduction

2022 Fudan

MS2 Coat Protein (MCP) is a capsid protein that self-assembles to a capsid. The protein can bind to the MS2-stemloop and acts as an adapter for RNA binding to the interest protein.[1]

Dimer MCP was used in TEARS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 283


References

  1. ↑ Guo, Haotian et al. “Spatial engineering of E. coli with addressable phase-separated RNAs.” Cell vol. 185,20 (2022): 3823-3837.e23. doi:10.1016/j.cell.2022.09.016