Difference between revisions of "Part:BBa K4158011"

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This part contains RBS and GFP coding site. Especially, used for construction of the plasmid PedcR-RBS-GFP, our project device with PedcR (<partinfo>BBa_K4158013</partinfo>).
 
This part contains RBS and GFP coding site. Especially, used for construction of the plasmid PedcR-RBS-GFP, our project device with PedcR (<partinfo>BBa_K4158013</partinfo>).
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Information of the sequence was from previous paper[1].
  
  
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===References===
 
===References===
1. Sankar K et al. A progesterone biosensor derived from microbial screening. <i>ACS Sens</i>. <b>7</b>(4):1132-1137(2022).
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1. Ibero J et al. Identification of the EdcR Estradiol-Dependent Repressor in Caenibius tardaugens NBRC 16725: Construction of a Cellular Estradiol Biosensor. Genes (Basel). Genes (Basel). 12(12):1846(2022).

Revision as of 15:28, 12 October 2022


RBS-GFP

This part contains RBS and GFP coding site. Especially, used for construction of the plasmid PedcR-RBS-GFP, our project device with PedcR (BBa_K4158013).

Information of the sequence was from previous paper[1].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 658


References

1. Ibero J et al. Identification of the EdcR Estradiol-Dependent Repressor in Caenibius tardaugens NBRC 16725: Construction of a Cellular Estradiol Biosensor. Genes (Basel). Genes (Basel). 12(12):1846(2022).