Difference between revisions of "Part:BBa K4439009"

Latest revision as of 14:59, 12 October 2022

GST-N[AS]8C-CBD-10xHis

Abstract

The GST-N[AS]8C-CBD-10xHis protein was implemented in a pET28a vector. This protein could allow to test the polymerisation of [AS] modules (Part:BBa K3944000) present in recombinant green lacewing silk proteins N[AS]8C (Part:BBa K4439002) to create a very specific binding between two proteins.

The assembly compatibility of this part surprised the team: we think that we don't have any assembly compatibility because of the fact that we did a small mutation on the CBD sequence that is not taken into account in the part we used (Part:BBa K1321014).

The mutation is at bp153 where a C was changed into a T, which is a silent mutation.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 2592
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2592
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2592
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 2592
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 2592
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 82

@@ Line 2: / Line 2: @@
 ==Abstract==
-The GST-N[AS]8C-CBD-10xHis protein was implemented in a pET28a vector. This protein could allow to test the polymerisation of [AS] modules ([[Part:BBa K3944000]]) present in recombinant green lacewing silk proteins ([[Part:BBa K4439002]]) to create a very specific binding between two proteins.
+The GST-N[AS]8C-CBD-10xHis protein was implemented in a pET28a vector. This protein could allow to test the polymerisation of [AS] modules ([[Part:BBa K3944000]]) present in recombinant green lacewing silk proteins N[AS]8C ([[Part:BBa K4439002]]) to create a very specific binding between two proteins.
 The assembly compatibility of this part surprised the team: we think that we don't have any assembly compatibility because of the fact that we did a small mutation on the CBD sequence that is not taken into account in the part we used ([[Part:BBa K1321014]]).