Difference between revisions of "Part:BBa K4438707"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | The part P4G13_trigger_4_phi29(BBa_K4438707) has complete complementarity to the part P4G13_aptamer (BBa_K4438700) and blocks a part of the binding domain of the aptamer. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. Progesterone binds with P4G13_aptamer (BBa_K4438700) with high affinity and displaces the P4G13_trigger_4_phi29(BBa_K4438707) [1]. | + | The part P4G13_trigger_4_phi29(<partinfo>BBa_K4438707</partinfo>) has complete complementarity to the part P4G13_aptamer (<partinfo>BBa_K4438700</partinfo>) and blocks a part of the binding domain of the aptamer. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. Progesterone binds with P4G13_aptamer (BBa_K4438700) with high affinity and displaces the P4G13_trigger_4_phi29(<partinfo>BBa_K4438707</partinfo>) [1]. |
| − | This part has complete complementarity with part P4G13_Target_4 (BBa_K4438708). Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. | + | This part has complete complementarity with part P4G13_Target_4 (<partinfo>BBa_K4438708</partinfo>). Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. |
Different levels of progesterone can be detected using all these three parts. | Different levels of progesterone can be detected using all these three parts. | ||
| + | https://parts.igem.org/File:T--IISER-Tirupati_India--Pg413_4.png | ||
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Revision as of 13:39, 12 October 2022
P4G13_trigger_4_phi29
P4G13_trigger_4_phi29(BBa_K4438707) is single-stranded DNA having 15 nucleotides. Figure 1B) shows the secondary structure and minimum free energy.
Usage and Biology
The part P4G13_trigger_4_phi29(BBa_K4438707) has complete complementarity to the part P4G13_aptamer (BBa_K4438700) and blocks a part of the binding domain of the aptamer. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. Progesterone binds with P4G13_aptamer (BBa_K4438700) with high affinity and displaces the P4G13_trigger_4_phi29(BBa_K4438707) [1]. This part has complete complementarity with part P4G13_Target_4 (BBa_K4438708). Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. Different levels of progesterone can be detected using all these three parts.
https://parts.igem.org/File:T--IISER-Tirupati_India--Pg413_4.png
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Contreras Jiménez, G., Eissa, S., Ng, A., Alhadrami, H., Zourob, M., & Siaj, M. (2015). Aptamer-based label-free impedimetric biosensor for detection of progesterone. Analytical chemistry, 87(2), 1075-1082.