Difference between revisions of "Part:BBa K4438710"
 
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===Usage and Biology===
 
===Usage and Biology===
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This part along with P4G13_trigger_5_phi29 (<partinfo>BBa_K4438709</partinfo) and P4G13_aptamer (<partinfo>BBa_K4438700</partinfo>) can be used for sensing different concentrations of progesterone [1].
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This part along with P4G13_trigger_5_phi29 (<partinfo>BBa_K4438709</partinfo>) and P4G13_aptamer (<partinfo>BBa_K4438700</partinfo>) can be used for sensing different concentrations of progesterone [1].
  
  

Latest revision as of 13:21, 12 October 2022

P4G13_Target_5

P4G13_Target_5 (BBa_K4438710) is a single-stranded DNA sequence having 100 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 5’ end has a few bases complementary to the P4G13_trigger_5_phi29 (BBa_K4438709). The middle region has ssDNA sense T7 promoter sequence. The 3’ end has an ssDNA sense broccoli sequence.

Usage and Biology

This part along with P4G13_trigger_5_phi29 (BBa_K4438709) and P4G13_aptamer (BBa_K4438700) can be used for sensing different concentrations of progesterone [1].


T--IISER-Tirupati India--Pg413 5.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Contreras Jiménez, G., Eissa, S., Ng, A., Alhadrami, H., Zourob, M., & Siaj, M. (2015). Aptamer-based label-free impedimetric biosensor for detection of progesterone. Analytical chemistry, 87(2), 1075-1082.