Difference between revisions of "Part:BBa K4115046"
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This Composite part is integrated into the plasmid backbone of PUC57 and entered <i>Synechococcus</i> HL7942 by natural transformation with <i>E. coli</i>. LuxR is a common receptor protein in bacterial quorum sensing. The LuxR in this composite part plays a role in receiving and responding to AHL(3O C6 HSL) signal molecules [1]. When LuxR receives the signal of AHL, it can regulate the downstream Lux pR [https://parts.igem.org/Part:BBa_R0062 (BBa_R0062)] to make superfolder GFP [https://parts.igem.org/Part:BBa_K4115000 (BBa_K4115000)] express.(More detailed infomation can be seen at the composite part [https://parts.igem.org/Part:BBa_K4115039 (BBa_K4115039)])<br>  
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This Composite part is integrated into the plasmid backbone of PUC57 and entered <i>Synechococcus</i> HL7942 by natural transformation with <i>E. coli</i>. LuxR is a common receptor protein in bacterial quorum sensing. The LuxR in this composite part plays a role in receiving and responding to AHL(3O C6 HSL) signal molecules [1]. When LuxR receives the signal of AHL, it can regulate the downstream Lux pR [https://parts.igem.org/Part:BBa_R0062 (BBa_R0062)] to make superfolder GFP [https://parts.igem.org/Part:BBa_K4115000 (BBa_K4115000)] express.(More detailed infomation can be seen at the composite part [https://parts.igem.org/Part:BBa_K4115039 BBa_K4115039])<br>  
 
We can construct a communication and regulation system between <i>E.coli</i> and <i>S.elongatus</i> HL7942<br> with Lux network.
 
We can construct a communication and regulation system between <i>E.coli</i> and <i>S.elongatus</i> HL7942<br> with Lux network.
  

Revision as of 15:29, 11 October 2022


NS3-2-KanR-J23101-B0034-LuxR-B0015-Lux pR-B0034-sfGFP-B0015-NS3-1

NS3-2-lac UV5 promoter-cscB-lacI-KanR-NS3-1
Function Gene regulator
Use in S.elongatus HL7942
RFC standard None
Backbone pUC57
Submitted by ShanghaiTech_China

This Composite part is integrated into the plasmid backbone of PUC57 and entered Synechococcus HL7942 by natural transformation with E. coli. LuxR is a common receptor protein in bacterial quorum sensing. The LuxR in this composite part plays a role in receiving and responding to AHL(3O C6 HSL) signal molecules [1]. When LuxR receives the signal of AHL, it can regulate the downstream Lux pR (BBa_R0062) to make superfolder GFP (BBa_K4115000) express.(More detailed infomation can be seen at the composite part BBa_K4115039)
We can construct a communication and regulation system between E.coli and S.elongatus HL7942
with Lux network.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 507
    Illegal PstI site found at 4261
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1167
    Illegal NheI site found at 1190
    Illegal PstI site found at 507
    Illegal PstI site found at 4261
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 507
    Illegal PstI site found at 4261
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 507
    Illegal PstI site found at 4261
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2145

References

[1] Kylilis, N., Tuza, Z.A., Stan, GB. et al. Tools for engineering coordinated system behaviour in synthetic microbial consortia. Nat Commun 9, 2677 (2018). https://doi.org/10.1038/s41467-018-05046-2