Difference between revisions of "Part:BBa K4244060"
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<partinfo>BBa_K4244060 short</partinfo> | <partinfo>BBa_K4244060 short</partinfo> | ||
| − | + | sfGFP <partinfo>BBa_K4244004</partinfo> was fused to OmpA <partinfo>BBa_K208003</partinfo> to secrete sfGFP and use it as an extracellular reporter. It is under an constitutive medium strength promoter <partinfo>BBa_K4244057</partinfo>. | |
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| + | ====Secretion assay==== | ||
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| + | [[File:BBa K4244060-OmpA ts.png|thumb|500px|center|<font size="1">Figure 1: Time series measurement of total, pellet and supernatant fraction of sfGFP under the OmpA signal peptide expressed with constitutive promoter. Measurement was performed every two hours for eight hours. No significant fluorescence was detected in the supernatant fraction.</font>]] | ||
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| + | ====Discussion==== | ||
| + | No secretion was observed in ''E. coli'' Nissle. This may be due to the too high expression level of sfGFP under <partinfo>BBa_K4244057</partinfo>. This experiment still need adjustment and fine tuning to prove the secretion of sfGFP. | ||
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Revision as of 11:32, 10 October 2022
Constitutive expression of sfGFP with OmpA secretion tag
sfGFP BBa_K4244004 was fused to OmpA BBa_K208003 to secrete sfGFP and use it as an extracellular reporter. It is under an constitutive medium strength promoter BBa_K4244057.
Secretion assay
Discussion
No secretion was observed in E. coli Nissle. This may be due to the too high expression level of sfGFP under BBa_K4244057. This experiment still need adjustment and fine tuning to prove the secretion of sfGFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]