Difference between revisions of "Part:BBa K4214002"
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<partinfo>BBa_K4214002 short</partinfo> | <partinfo>BBa_K4214002 short</partinfo> | ||
| − | Human GIF with His6-tag. This part has the signal peptide | + | Human GIF with His6-tag. This part has the native signal peptide removed, as it is not required for bacterial expression or in our mammalian expression vector. This part was used to produce and purify our Human GIF in bacteria, through the composite parts BBa_K421403 (with LacI promotor) and BBa_K421404 (with LacI promotor and pelB leader sequence). |
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| + | =Usage and Biology= | ||
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| + | *NCBI page of the human gastric intrinsic factor [https://www.ncbi.nlm.nih.gov/gene/2694] | ||
| + | *This part of hGIF does not contain the native signal peptide (AA1-18) | ||
| + | *This part was used in parts BBa_K421403[https://parts.igem.org/Part:BBa_K4214003] and BBa_K421404[https://parts.igem.org/Part:BBa_K4214004] | ||
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Revision as of 12:09, 9 October 2022
hGIF with 6xHis-tag
Human GIF with His6-tag. This part has the native signal peptide removed, as it is not required for bacterial expression or in our mammalian expression vector. This part was used to produce and purify our Human GIF in bacteria, through the composite parts BBa_K421403 (with LacI promotor) and BBa_K421404 (with LacI promotor and pelB leader sequence).
Usage and Biology
- NCBI page of the human gastric intrinsic factor [1]
- This part of hGIF does not contain the native signal peptide (AA1-18)
- This part was used in parts BBa_K421403[2] and BBa_K421404[3]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 257
Illegal BsaI.rc site found at 937
Illegal SapI site found at 927