Difference between revisions of "Part:BBa K4214000"

Revision as of 08:54, 9 October 2022

Human Gastric Intrinsic Factor (hGIF)

This part codes for human Gastric Intrinsic Factor protein (AA19-417). The gene is located in chromosome 11q12.1. This gene is a part of cobalamin transporters group. It is essential for the absorption of vitamin B12. Mutations in this gene may lead to pernicious anemia.

We removed the native signal peptide (AA1-18) from the N-terminal in order to obtain a sequence that can be worked with more easily in bacterial systems. However, since we used bacterial and mammalian expression vectors in parallel, we needed to have compatible parts and decided to work only with part BBa_K4214001[1], which includes the signal peptide sequence (AA1-18).

[[Image:File:File:Registry_IF.png |thumb|450px|right|Fig. : Human GIF (BBa_K4214000) ]]

Usage and Biology

NCBI page of the human gastric intrinsic factor [2]
We designed this biobrick in order to have a sequence for our other parts
This part of hGIF should be mentioned that does not contain the signal peptide

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 265
Illegal BsaI.rc site found at 945
Illegal SapI site found at 935

@@ Line 7: / Line 7: @@
 We '''removed''' the native signal peptide (AA1-18) from the N-terminal in order to obtain a sequence that can be worked with more easily in bacterial systems. However, since we used bacterial and mammalian expression vectors in parallel, we needed to have compatible parts and decided to work only with '''part BBa_K4214001'''[https://parts.igem.org/Part:BBa_K4214001], which '''includes''' the signal peptide sequence (AA1-18).
-[[Image:File:Registry IF.png
+[[Image:File:File:Registry_IF.png
 |thumb|450px|right|Fig. : ''Human GIF (BBa_K4214000) '' ]]
 ==Usage and Biology==