Difference between revisions of "Part:BBa K4214001"
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*This new part can be found here. (link for new part )
 
*This new part can be found here. (link for new part )
  
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[[https://static.igem.wiki/teams/4214/wiki/results/wtif-gblock-in-agarose-gel.png|thumb|450px|right|Fig. 1: ''The wtIF gene from gblock was amplified by using two different set of primers '' ]]
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[[Image: https://static.igem.wiki/teams/4214/wiki/results/wtif-gblock-in-agarose-gel.png|thumb|450px|right|Fig. 1: ''The wtIF gene from gblock was amplified by using two different set of primers '' ]]
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4214001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4214001 SequenceAndFeatures</partinfo>

Revision as of 08:00, 9 October 2022

Human Gastric Intrinsic Factor (hGIF)


This part codes for human Gastric Intrinsic Factor. We used the human sequence of the gene. The gene is located in chromosome 11q12.1. This gene is a part of cobalamin transporters group. It is essential for the absorption of vitamin B12. Mutations in this gene may lead to pernicious anemia.


Usage and Biology

  • NCBI page of the human gastric intrinsic factor [1]
  • We designed this biobrick in order to use this coding region for generating our mutants so that we ensure the evading of the autoantibodies of pernicious anemia
  • We used this part with some extra modifications to perform protein surface display in mammalian cells but also protein production in E.coli
  • This new part can be found here. (link for new part )
File:Https://static.igem.wiki/teams/4214/wiki/results/wtif-gblock-in-agarose-gel.png
Fig. 1: The wtIF gene from gblock was amplified by using two different set of primers

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 21
    Illegal SpeI site found at 94
    Illegal PstI site found at 1316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 94
    Illegal PstI site found at 1316
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 21
    Illegal SpeI site found at 94
    Illegal PstI site found at 1316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 21
    Illegal SpeI site found at 94
    Illegal PstI site found at 1316
    Illegal NgoMIV site found at 14
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 353
    Illegal BsaI.rc site found at 1033
    Illegal SapI site found at 1023