Difference between revisions of "Part:BBa K4156125"

 
 
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<partinfo>BBa_K4156125 short</partinfo>
 
<partinfo>BBa_K4156125 short</partinfo>
  
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OR gate-HlyE consists of an OR logic gate (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156123"> BBa_K4156123 </a></html>) and a downstream HlyE (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156084"> BBa_K4156084 </a></html>) sequence, and tests the performance of the OR gate by detecting the red fluorescent signal.
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<!-- Add more about the biology of this part here -->
  
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===Usage and Biology===
 
===Usage and Biology===
  
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The construction of this part is similar to OR gate-mRFP (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156124"> BBa_K4156124 </a></html>). The difference is that we replaced the mRFP protein with the HlyE therapeutic protein. This is to verify the therapeutic power of the recombinant strain under OR gate control. After co-incubation of the recombinant strain with cancer cells, cell viability was monitored using the CCK8 assay to assess the therapeutic power of the recombinant strain and the reinforcing effect of the OR gate.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4156125 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4156125 SequenceAndFeatures</partinfo>

Latest revision as of 07:51, 9 October 2022


OR gate-HlyE

OR gate-HlyE consists of an OR logic gate ( BBa_K4156123 ) and a downstream HlyE ( BBa_K4156084 ) sequence, and tests the performance of the OR gate by detecting the red fluorescent signal.


Usage and Biology

The construction of this part is similar to OR gate-mRFP ( BBa_K4156124 ). The difference is that we replaced the mRFP protein with the HlyE therapeutic protein. This is to verify the therapeutic power of the recombinant strain under OR gate control. After co-incubation of the recombinant strain with cancer cells, cell viability was monitored using the CCK8 assay to assess the therapeutic power of the recombinant strain and the reinforcing effect of the OR gate.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 104
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 124
    Illegal BsaI site found at 306