Difference between revisions of "Part:BBa K182005:Design"
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===Design Notes=== | ===Design Notes=== | ||
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The cloning involved ligating K182004 (upstream) and S03518 (downstream) together into an Ampicillin / Chloramphenicol vector (pSB1AC3), creating the BioBrick named K182005. K182005 was then tested using BB_I13600, by transforming them both into a cell and observing the behaviour of the CFP expression. | The cloning involved ligating K182004 (upstream) and S03518 (downstream) together into an Ampicillin / Chloramphenicol vector (pSB1AC3), creating the BioBrick named K182005. K182005 was then tested using BB_I13600, by transforming them both into a cell and observing the behaviour of the CFP expression. | ||
Latest revision as of 21:32, 23 September 2009
TetR regulated by CI operator (RBS+, Term-) with LVA tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The cloning involved ligating K182004 (upstream) and S03518 (downstream) together into an Ampicillin / Chloramphenicol vector (pSB1AC3), creating the BioBrick named K182005. K182005 was then tested using BB_I13600, by transforming them both into a cell and observing the behaviour of the CFP expression.
Source
BBa_K182004 was used as the upstream part, BBa_S03518 was used as the downstream part. pSB1AC3 was our recipient vector.