Difference between revisions of "Part:BBa K182005:Design"

(→‎Design Notes)
(→‎Design Notes)
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[[Image:Laci cloning step 1Aberdeen2009.png|center|400 px]]
 
[[Image:Laci cloning step 1Aberdeen2009.png|center|400 px]]
  
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The cloning involved ligating K182004 (upstream) and S03518 (downstream) together into a Chloramphenicol vector, creating the BioBrick named K182005. K182005 was then tested using BB_I13600, by transforming them both into a cell and observing the behaviour of the CFP expression.
+
The cloning involved ligating K182004 (upstream) and S03518 (downstream) together into a ampicillin/Chloramphenicol vector (PSB1AC3), creating the BioBrick named K182005. K182005 was then tested using BB_I13600, by transforming them both into a cell and observing the behaviour of the CFP expression.
  
 
===Source===
 
===Source===

Revision as of 12:39, 23 September 2009

TetR regulated by CI operator (RBS+, Term-) with LVA tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Laci cloning step 1Aberdeen2009.png

The cloning involved ligating K182004 (upstream) and S03518 (downstream) together into a ampicillin/Chloramphenicol vector (PSB1AC3), creating the BioBrick named K182005. K182005 was then tested using BB_I13600, by transforming them both into a cell and observing the behaviour of the CFP expression.

Source

BBa_K182004 was used as the upstream part, BBa_S03518 was used as the downstream part. pSB1AC3 was our recipient vector.

References