Difference between revisions of "Part:BBa K4197019"

Revision as of 22:25, 8 October 2022 (view source) Charline baraban (Talk | contribs) ← Older edit		Latest revision as of 22:29, 8 October 2022 (view source) Charline baraban (Talk | contribs)
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	<h2>References</h2>		<h2>References</h2>
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		+	<p>More information about the project for which the part was created:<a href="https://2022.igem.wiki/toulouse-insa-ups/index.html"> DAISY (INSA-UPS 2022)</a> </p>
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		+	<p>Other parts to display allergens:<br>
		+	<p>Other parts using the DARPin:<br>
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		+	- <a href="https://parts.igem.org/Part:BBa_K4197011">OmpA_DARPin</a><br>
		+	- <a href="https://parts.igem.org/Part:BBa_K4197010">OmpA_DARPin_sfGFP</a><br>
		+	- <a href="https://parts.igem.org/Part:BBa_K4197001"> OmpA_DARPin fusion + ihfB800-mTagBFP</a><br>
		+	</p>
	<ol>		<ol>
	<i>		<i>

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Latest revision as of 22:29, 8 October 2022

DARPin e2_79

Gene coding for DARPin e2_79 which is a protein that recognises the constant part of IgE (Baumann et al., 2010).

Introduction

This part is composed of the gene coding for the DARPin E2_79 protein. DARPin is a protein that recognizes the constant part of IgE (Baumann et al., 2010). It has already been expressed in E. coli

Construction

The objective of the INSA-UPS 2022 team was to display DARPin E2_79 on the surface of E. coli so the ordered sequence was merged to OmpA membrane lipoprotein (see part BBa_K1694011).

References

More information about the project for which the part was created: DAISY (INSA-UPS 2022)

Other parts to display allergens:

Other parts using the DARPin:
- OmpA_DARPin
- OmpA_DARPin_sfGFP
- OmpA_DARPin fusion + ihfB800-mTagBFP

1. Baumann, M. J., Eggel, A., Amstutz, P., Stadler, B. M., & Vogel, M. (2010). DARPins against a functional IgE epitope. Immunology Letters, 133(2), 78–84. https://doi.org/10.1016/j.imlet.2010.07.005

Sequence and Features